A Novel Sensitive Immunoassay Targeting the 5-Methylthio- d -Xylofuranose–Lipoarabinomannan Epitope Meets the WHO's Performance Target for Tuberculosis Diagnosis-Reference-Cited by-同舟云学术

A Novel Sensitive Immunoassay Targeting the 5-Methylthio- d -Xylofuranose–Lipoarabinomannan Epitope Meets the WHO's Performance Target for Tuberculosis Diagnosis

Published:2018-12 Issue:12 Volume:56 Page:
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Sigal George B.¹,Pinter Abraham²,Lowary Todd L.³,Kawasaki Masanori⁴,Li Andra⁵,Mathew Anu¹,Tsionsky Michael¹,Zheng Ruixiang Blake³,Plisova Tatiana¹,Shen Ke³,Katsuragi Kiyonori⁴,Choudhary Alok²,Honnen William J.²,Nahid Payam⁶,Denkinger Claudia M.⁷,Broger Tobias⁷^ORCID

Affiliation:

1. Meso Scale Diagnostics, LLC, Rockville, Maryland, USA

2. Public Health Research Institute Center, New Jersey Medical School, Rutgers University, Newark, New Jersey, USA

3. Department of Chemistry, Alberta Glycomics Centre, University of Alberta, Edmonton, AB, Canada

4. Otsuka Pharmaceutical Co., Ltd., Tokyo, Japan

5. Immunoprecise Antibodies Ltd., Victoria, British Columbia, Canada

6. Division of Pulmonary and Critical Care Medicine, University of California, San Francisco, San Francisco, California, USA

7. FIND, Geneva, Switzerland

Abstract

The only currently commercialized point-of-care assay for tuberculosis (TB) that measures lipoarabinomannan (LAM) in urine (Alere LF-LAM) has insufficient sensitivity. We evaluated the potential of 100 novel monoclonal antibody pairs targeting a variety of LAM epitopes on a sensitive electrochemiluminescence platform to improve the diagnostic accuracy.

Funder

Global Health Innovative Technology Fund

UK Department for International Deelopment

Dutch Ministry of Foreign Affairs

HHS | National Institutes of Health

HHS | NIH | National Institute of Allergy and Infectious Diseases

Alberta Glycomics Centre

Bill and Melinda Gates Foundation

Publisher

American Society for Microbiology

Subject

Microbiology (medical)