Validation of Bartonella henselae Western Immunoblotting for Serodiagnosis of Bartonelloses in Dogs-Reference-Cited by-同舟云学术

Validation of Bartonella henselae Western Immunoblotting for Serodiagnosis of Bartonelloses in Dogs

Published:2020-03-25 Issue:4 Volume:58 Page:
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Neupane Pradeep¹²,Sevala Sindhura¹²,Balakrishnan Nandhakumar³,Marr Henry¹²,Wilson James¹²,Maggi Ricardo¹²,Birkenheuer Adam¹²,Lappin Michael⁴,Chomel Bruno⁵,Breitschwerdt Edward B.¹²

Affiliation:

1. Department of Clinical Sciences, College of Veterinary Medicine, North Carolina State University, Raleigh, North Carolina, USA

2. Intracellular Pathogens Research Laboratory, Comparative Medicine Institute, College of Veterinary Medicine, North Carolina State University, Raleigh, North Carolina, USA

3. Bureau of Laboratories, Division of Infectious Disease, Michigan Department of Health and Human Services, Lansing, Michigan, USA

4. Department of Clinical Sciences, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, Fort Collins, Colorado, USA

5. Department of Population Health & Reproduction, School of Veterinary Medicine, University of California, Davis, California, USA

Abstract

Bartonella spp. are etiological agents of life-threatening zoonotic diseases in dogs worldwide. Due to the poor sensitivity of immunofluorescent-antibody assays (IFAs), a reliable serodiagnostic test for canine bartonelloses is of clinical importance. The utility of Western blotting (WB) for the serodiagnosis of canine bartonelloses has not been critically investigated. The objective of this study was to characterize WB immunodominant proteins that could be used to confirm a serodiagnosis of bartonelloses.

Funder

American Kennel Club Canine Health Foundation

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.01335-19

Reference29 articles.

1. Bartonellosis: One Health Perspectives for an Emerging Infectious Disease

2. Bartonellosis, One Health and all creatures great and small

3. Bartonella, a Common Cause of Endocarditis: a Report on 106 Cases and Review