Affiliation:
1. Department of Microbiology, The University of Chicago, Chicago, Illinois 60637
Abstract
Herpes simplex virus subtype 1 deoxyribonucleic acid (DNA) was sheared in a French press to uniform fragments, denatured by heating, then allowed to reassociate. The renaturation reaction followed second-order kinetics with a single rate constant indicating that at least 95% of the genome was unique and that repetitive sequences, if present, were not detectable by this technique. The kinetic complexity of the herpes simplex genome was determined by DNA renaturation kinetics to be (95 ± 1) × 10
6
daltons. Since this value is in excellent agreement with the molecular weight of viral DNA [(99 ± 5) × 10
6
daltons] obtained from velocity sedimentation studies, it is concluded that virions contain only one species of double-stranded DNA molecules 95 × 10
6
to 99 × 10
6
daltons in molecular weight.
Publisher
American Society for Microbiology
Subject
Virology,Insect Science,Immunology,Microbiology
Reference9 articles.
1. Herpes simplex virus DNA;Becker Y.;Virology,1968
2. Nucleotide sequence repetition in DNA;Britten R. J.;Yearbook Carnegie Inst.,1967
3. Repeated sequences in DNA;Britten R. J.;Science,1968
4. A bovine genome;Britten R. J.;Yearbook Carnegie Inst.,1970
5. The determination of molecular weight of bacterial genome DNA from renaturation rates;Gillis M.;Eur. J. Biochem.,1970
Cited by
47 articles.
订阅此论文施引文献
订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献