The Gene CBO0515 from Clostridium botulinum Strain Hall A Encodes the Rare Enzyme N 5 -(Carboxyethyl) Ornithine Synthase, EC 1.5.1.24-Reference-Cited by-同舟云学术

The Gene CBO0515 from Clostridium botulinum Strain Hall A Encodes the Rare Enzyme N 5 -(Carboxyethyl) Ornithine Synthase, EC 1.5.1.24

Published:2010-02-15 Issue:4 Volume:192 Page:1151-1155
ISSN:0021-9193
Container-title:Journal of Bacteriology
language:en
Short-container-title:J Bacteriol

Author:

Thompson John¹,Hill Karen K.²,Smith Theresa J.³,Pikis Andreas¹⁴

Affiliation:

1. Microbial Biochemistry and Genetics Unit, Oral Infection and Immunity Branch, NIDCR, National Institutes of Health, DHHS, Bethesda, Maryland 20892

2. Bioscience Division, MS:M888, Los Alamos National Laboratory, Los Alamos, New Mexico 87545

3. Integrated Toxicology Division, U.S. Army Medical Institute of Infectious Diseases, Fort Detrick, Maryland 21702

4. Center for Drug Evaluation and Research, Food and Drug Administration, Silver Spring, Maryland 20993

Abstract

ABSTRACT Sequencing of the genome of Clostridium botulinum strain Hall A revealed a gene (CBO0515), whose putative amino acid sequence was suggestive of the rare enzyme N 5 -(1-carboxyethyl) ornithine synthase. To test this hypothesis, CBO0515 has been cloned, and the encoded polypeptide was purified and characterized. This unusual gene appears to be confined to proteolytic strains assigned to group 1 of C. botulinum .

Publisher

American Society for Microbiology

Subject

Molecular Biology,Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/JB.01044-09

Reference20 articles.

1. Gapped BLAST and PSI-BLAST: a new generation of protein database search programs

2. Arnon, S. S., R. Schechter, T. V. Inglesby, D. A. Henderson, J. G. Bartlett, M. S. Ascher, E. Eitzen, A. D. Fine, J. Hauer, M. Layton, S. Lillibridge, M. T. Osterholm, T. O'Toole, G. Parker, T. M. Perl, P. K. Russell, D. L. Swerdlow, and K. Tonat. 2001. Botulinum toxin as a biological weapon: medical and public health management. JAMA 285 : 1059-1070.

3. Baker, P. J., Y. Sawa, H. Shibata, S. E. Sedelnikova, and D. W. Rice. 1998. Analysis of the structure and substrate binding of Phormidium lapideum alanine dehydrogenase. Nat. Struct. Biol. 5 : 561-567.

4. Independent evolution of neurotoxin and flagellar genetic loci in proteolytic Clostridium botulinum

5. Collins, M. D., and A. K. East. 1998. Phylogeny and taxonomy of the food-borne pathogen Clostridium botulinum and its neurotoxins. J. Appl. Microbiol. 84 : 5-17.