Affiliation:
1. Department of Vaccine Production, National Bacteriological Laboratory, Stockholm, Sweden.
Abstract
Hybrid cell lines producing monoclonal antibodies against Bordetella pertussis filamentous hemagglutinin (FHA) were established. The specificity of the antibodies was ascertained by enzyme-linked immunosorbent assay (ELISA), sandwich ELISA, and sodium dodecyl sulfate-polyacrylamide gel electrophoresis followed by electroblotting. The monoclonal antibody-based sandwich ELISA was developed for detection of B. pertussis FHA. The assay had a detection limit of B. pertussis FHA in concentrations ranging from 7 to 15 ng/ml. The assay was also able to detect whole B. pertussis, Bordetella parapertussis, and Bordetella bronchiseptica bacteria. No cross-reactions were observed with strains of Branhamella catarrhalis, Neisseria meningitidis, Haemophilus influenzae, Klebsiella pneumoniae, Legionella pneumophila, Streptococcus miteor, or Streptococcus pneumoniae. The monoclonal antibodies might be useful for the detection of soluble antigens and whole bacteria in clinical samples and for studies of the immunochemical structure of B. pertussis FHA.
Publisher
American Society for Microbiology
Reference28 articles.
1. Characterization of the filamentous hemagglutinin from Bordetella pertussis by gel electrophoresis;An der Lan B.;Mol. Cell. Biochem.,1986
2. Separation and characterization of two distinct hemagglutinins contained in purified leukocytosispromoting factor from Bordetella pertussis;Arai H.;Biochim. Biophys. Acta,1976
3. Purification and characterisation of a fimbrial hemagglutinin from Bordetella pertussis for use in an enzyme-linked immunosorbent assay;Askelof P.;J. Med. Microbiol.,1982
4. Cloning of the filamentous hemagglutinin of Bordetella pertussis and its expression in Escherichia coli;Brown D. R.;Infect. Immun.,1987
5. Cowell J. L. Y. Sato H. Sato B. An der Lan and C. R. Manclark. 1982. Separation purification and properties of the filamentous hemagglutinin and the leucocytosis promoting factor-hemagglutinin from Bordetella pertussis p. 371-379. In J. B. Robbins J. C. Hill and J. C. Sadoff (ed.) Bacterial vaccines. Thieme-Stratton Inc. New York.
Cited by
7 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献