Duplex PCR Assay Simultaneously Detecting and Differentiating Bartonella quintana , B . henselae , and Coxiella burnetii in Surgical Heart Valve Specimens-Reference-Cited by-同舟云学术

Duplex PCR Assay Simultaneously Detecting and Differentiating Bartonella quintana , B . henselae , and Coxiella burnetii in Surgical Heart Valve Specimens

Published:2009-08 Issue:8 Volume:47 Page:2647-2650
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Tang Yi-Wei¹

Affiliation:

1. Departments of Pathology and Medicine, Vanderbilt University Medical Center, Nashville, Tennessee

Abstract

ABSTRACT A duplex PCR (dPCR) assay was developed to simultaneously detect and differentiate Bartonella quintana , Bartonella henselae , and Coxiella burnetii from surgical heart valve tissue specimens with an analytic sensitivity of 10 copies/reaction. Among 17 specimens collected from patients with a clinical diagnosis of culture-negative endocarditis, 2, 4, and 2 were positive for B . quintana , B . henselae , and C . burnetii , respectively, by the dPCR assay, which matched the results obtained by universal bacterial 16S rRNA gene amplification and sequencing.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.00721-09

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