A Little Gene with Big Effects: a
serT
Mutant Is Defective in
flgM
Gene Translation
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Published:2006-01
Issue:1
Volume:188
Page:297-304
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ISSN:0021-9193
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Container-title:Journal of Bacteriology
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language:en
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Short-container-title:J Bacteriol
Author:
Chevance Fabienne F. V.1, Karlinsey Joyce E.2, Wozniak Christopher E.12, Hughes Kelly T.1
Affiliation:
1. Department of Biology, University of Utah, Salt Lake City, Utah 84112 2. Department of Microbiology, Box 357242, University of Washington, Seattle, Washington 98195
Abstract
ABSTRACT
A conditional-lethal mutant was isolated as having a flagellar regulatory phenotype at 30°C and being unable to grow at 42°C. Chromosomal mapping localized the mutation to the
serT
gene, which encodes an essential serine tRNA species (
\batchmode \documentclass[fleqn,10pt,legalpaper]{article} \usepackage{amssymb} \usepackage{amsfonts} \usepackage{amsmath} \pagestyle{empty} \begin{document} \(\mathbf{tRNA}_{_{cmo}5\mathbf{UGA}}^{Ser}\) \end{document}
). DNA sequence analysis revealed the mutation to be a single base change in G:A at position 10 of the
serT
gene that lies within the D-stem of the essential
\batchmode \documentclass[fleqn,10pt,legalpaper]{article} \usepackage{amssymb} \usepackage{amsfonts} \usepackage{amsmath} \pagestyle{empty} \begin{document} \(\mathbf{tRNA}_{_{cmo}5}\mathbf{UGA}^{Ser}\) \end{document}
species.
\batchmode \documentclass[fleqn,10pt,legalpaper]{article} \usepackage{amssymb} \usepackage{amsfonts} \usepackage{amsmath} \pagestyle{empty} \begin{document} \(\mathbf{tRNA}_{_{cmo}5}\mathbf{UGA}^{Ser}\) \end{document}
recognizes UCA, UCG, and UCU codons, but UCU is also recognized by
\batchmode \documentclass[fleqn,10pt,legalpaper]{article} \usepackage{amssymb} \usepackage{amsfonts} \usepackage{amsmath} \pagestyle{empty} \begin{document} \(\mathbf{tRNA_{GGA}^{Ser}}\) \end{document}
and UCG by
\batchmode \documentclass[fleqn,10pt,legalpaper]{article} \usepackage{amssymb} \usepackage{amsfonts} \usepackage{amsmath} \pagestyle{empty} \begin{document} \(\mathbf{tRNA_{CGA}^{Ser}}\) \end{document}
. No other tRNAs are known to read the UCA codon. Thus, the UCA codon is specifically recognized by
\batchmode \documentclass[fleqn,10pt,legalpaper]{article} \usepackage{amssymb} \usepackage{amsfonts} \usepackage{amsmath} \pagestyle{empty} \begin{document} \(\mathbf{tRNA}_{_{cmo}5}\mathbf{UGA}^{Ser}\) \end{document}
. We show that the anti-σ
28
activity of FlgM is defective in the
serT
mutant strain. The
serT
allele causes a 10-fold increase in σ
28
-dependent
fliC
promoter transcription, indicating a defect in FlgM anti-σ
28
activity in the presence of the
serT
mutation. The
flgM
gene contains only one UCA codon. Changing the UCA of
flgM
to ACG reversed the effect of the
serT
allele. Implications for context effects in regulation of gene expression are discussed.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
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