Chlamydial Development Is Adversely Affected by Minor Changes in Amino Acid Supply, Blood Plasma Amino Acid Levels, and Glucose Deprivation

Author:

Harper Angela1,Pogson Christopher I.2,Jones Meirion L.1,Pearce John H.1

Affiliation:

1. Microbial Molecular Genetics and Cell Biology Group, School of Biological Sciences, University of Birmingham, Birmingham B15 2TT,1 and

2. Biochemical Sciences, Wellcome Research Laboratories, Beckenham, Kent BR3 3BS,2 United Kingdom

Abstract

ABSTRACT This study has demonstrated the extreme sensitivity of Chlamydia trachomatis growing in McCoy cells to small changes in external amino acid supply. In the absence of cycloheximide, a decrease in the amino acid concentration of medium to 75% of control values was sufficient to induce the growth of enlarged chlamydial forms of reduced infectivity. Morphology became more distorted and the yield of infectious particles from inclusions declined as medium amino acid levels were further reduced. These events correlated with a general decline in intracellular amino acids, as measured by high-performance liquid chromatography, suggesting that chlamydiae require a minimum concentration of each amino acid for normal development. Cycloheximide enhanced the production of normal organisms and increased infectivity yield in media, suggesting that the drug increased the available pool of amino acids. This was supported by intracellular amino acid analyses. Aberrant forms with reduced infectivity were also induced during supply of infected cell cultures with medium containing blood plasma amino acid concentrations, supporting the proposal that nutrient levels in vivo could promote abnormal chlamydial development. Markedly abnormal forms were also observed during glucose deprivation, providing further evidence that aberrant development is a general stress-related response.

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Immunology,Microbiology,Parasitology

Reference47 articles.

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3. A novel DNA-binding protein with regulatory and protective roles in starved Escherichia coli.

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5. Morphologic and antigenic characterization of interferon γ-mediated persistent Chlamydia trachomatis infection in vitro;Beatty W. L.;Proc. Natl. Acad. Sci. USA,1993

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