Using Superfolder Green Fluorescent Protein for Periplasmic Protein Localization Studies-Reference-Cited by-同舟云学术

Using Superfolder Green Fluorescent Protein for Periplasmic Protein Localization Studies

Published:2011-09-15 Issue:18 Volume:193 Page:4984-4987
ISSN:0021-9193
Container-title:Journal of Bacteriology
language:en
Short-container-title:J Bacteriol

Author:

Dinh Thuy¹,Bernhardt Thomas G.¹

Affiliation:

1. Department of Microbiology and Immunobiology, Harvard Medical School, 200 Longwood Avenue, Boston, Massachusetts 02115

Abstract

ABSTRACT Studies investigating the subcellular localization of periplasmic proteins have been hampered by problems with the export of green fluorescent protein (GFP). Here we show that a superfolding variant of GFP (sfGFP) is fluorescent following Sec-mediated transport and works best when the cotranslational branch of the pathway is employed.

Publisher

American Society for Microbiology

Subject

Molecular Biology,Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/JB.00315-11

Reference25 articles.

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