CrFK Feline Kidney Cells Produce an RD114-Like Endogenous Virus That Can Package Murine Leukemia Virus-Based Vectors

Author:

Baumann Jörg G.12,Günzburg Walter H.12,Salmons Brian3

Affiliation:

1. Institute of Virology, University of Veterinary Sciences, A-1210 Vienna, Austria,1and

2. Institute of Molecular Virology, GSF-Research Center for Environment and Health, D-85758 Oberschleissheim,2 and

3. Bavarian Nordic Research Institute, D-80807 Munich,3Germany

Abstract

ABSTRACT The feline kidney cell line CrFK is used extensively for viral infectivity assays and for study of the biology of various retroviruses and derived vectors. We demonstrate the production of an endogenous, RD114-like, infectious retrovirus from CrFK cells. This virus also is shown to efficiently package Moloney murine leukemia virus vectors.

Publisher

American Society for Microbiology

Subject

Virology,Insect Science,Immunology,Microbiology

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