Long-Term Rasamsonia argillacea Complex Species Colonization Revealed by PCR Amplification of Repetitive DNA Sequences in Cystic Fibrosis Patients

Author:

Mouhajir Abdelmounaim12,Matray Olivier34,Giraud Sandrine1,Mély Laurent5,Marguet Christophe6,Sermet-Gaudelus Isabelle7,Le Gal Solène18,Labbé Franck9,Person Christine10,Troussier Françoise11,Ballet Jean-Jacques3,Gargala Gilles34,Zouhair Rachid2,Bougnoux Marie-Elisabeth12,Bouchara Jean-Philippe113,Favennec Loïc34

Affiliation:

1. L'UNAM Université, Université d'Angers, Groupe d'Etude des Interactions Hôte-Pathogène, Angers, France

2. University Moulay Ismail, Faculty of Sciences, Meknes, Morocco

3. Laboratoire de Parasitologie-Mycologie, Centre Hospitalier Universitaire, Rouen, France

4. Normandie University, UNIROUEN, Rouen, France

5. Centre de Ressources et de Compétences en Mucoviscidose, Hôpital Renée Sabran, Giens, France

6. Centre de Ressources et de Compétences en Mucoviscidose, Service de Pédiatrie, Hôpital Charles Nicolle, Rouen, France

7. Centre de Ressources et de Compétences en Mucoviscidose, Unité de Pédiatrie, Hôpital Necker-Enfants Malades, Paris, France

8. Laboratoire de Parasitologie-Mycologie, Centre Hospitalier Universitaire, Brest, France

9. Laboratoire de Microbiologie, Hôpital Jacques Monod, Le Havre, France

10. Centre de Ressources et de Compétences en Mucoviscidose Adultes, Service de Pneumologie, Centre Hospitalier Universitaire, Angers, France

11. Centre de Ressources et de Compétences en Mucoviscidose Enfants, Service de Pédiatrie, Centre Hospitalier Universitaire, Angers, France

12. Laboratoire de Parasitologie-Mycologie, Centre Hospitalier Universitaire Necker-Enfants Malades, Paris, France

13. Laboratoire de Parasitologie-Mycologie, Centre Hospitalier Universitaire, Angers, France

Abstract

ABSTRACT The aim of this work was to document molecular epidemiology of Rasamsonia argillacea species complex isolates from cystic fibrosis (CF) patients. In this work, 116 isolates belonging to this species complex and collected from 26 CF patients and one patient with chronic granulomatous disease were characterized using PCR amplification assays of repetitive DNA sequences and electrophoretic separation of amplicons (rep-PCR). Data revealed a clustering consistent with molecular species identification. A single species was recovered from most patients. Rasamsonia aegroticola was the most common species, followed by R. argillacea sensu stricto and R. piperina , while R. eburnea was not identified. Of 29 genotypes, 7 were shared by distinct patients while 22 were patient specific. In each clinical sample, most isolates exhibited an identical genotype. Genotyping of isolates recovered from sequential samples from the same patient confirmed the capability of R. aegroticola and R. argillacea isolates to chronically colonize the airways. A unique genotype was recovered from two siblings during a 6-month period. In the other cases, a largely dominant genotype was detected. Present results which support the use of rep-PCR for both identification and genotyping for the R. argillacea species complex provide the first molecular evidence of chronic airway colonization by these fungi in CF patients.

Funder

Association Vaincre la Mucoviscidose

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

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