Staphylococcus aureus chromosomal mutations that decrease efficiency of Rep utilization in replication of pT181 and related plasmids

Author:

Iordanescu S1,Bargonetti J1

Affiliation:

1. Department of Plasmid Biology, Public Health Research Institute, New York, New York 10016.

Abstract

Staphylococcus aureus chromosomal mutants which maintain pT181 and related plasmids at a much reduced copy number but which do not affect the replication of other plasmids have been isolated. The origin of replication and the initiator protein of the affected plasmids are the only elements required for the response to these mutations. The host mutations do not interfere with the pT181 replication control mechanism.

Publisher

American Society for Microbiology

Subject

Molecular Biology,Microbiology

Reference19 articles.

1. Control of pT181 replication. II. Mutational analysis;Carleton S.;EMBO J.,1984

2. The rep mutation. II. Its effect on Escherichia coli and on the replication of bacteriophage 4X174;Denhardt D. T.;Virology,1972

3. The integration factor of Escherichia coli binds to multiple sites at plasmid R6K y origin and is essential for replication;Filutowicz M.;Nucleic Acids Res.,1988

4. Replication of pSC101: effects of mutations in the E. coli DNA binding protein IHF;Gamas P.;Mol. Gen. Genet.,1986

5. Integration host factor interacts with the DNA replication enhancer of filamentous phage fl;Greenstein D.;Proc. Natl. Acad. Sci. USA,1988

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