Affiliation:
1. Department of Biology, Georgetown University, Washington, D.C. 20007
Abstract
Polynucleotide relationships among selected
Vibrio
species were examined by means of deoxyribonucleic acid (DNA) reassociation reactions and chromatography on hydroxyapatite. Relative levels of intraspecific DNA duplex formation (
V. cholerae-V. cholerae
and
V. parahaemolyticus-V. parahaemolyticus
) were found to be high at 60 C (>80%), and only minimally reduced at 75 C. Interspecific DNA duplexes between
V. cholerae
DNA and that of the non-cholera vibrios also exhibited high relative levels of formation at 60 C (>80%) and, with one exception, were only slightly reduced at 75 C. The thermal stability of these duplexes formed at 60 or 75 C was virtually identical to that of homologous
V. cholerae
DNA duplexes. The degree of reassociation and the thermal stability of
V. cholerae
-non-cholera vibrio DNA duplexes suggests relatively little evolutionary divergence in these organisms. In all other interspecific DNA reassociation reactions, only low levels of DNA duplex formation were noted at 60 C (<25%), and these were drastically reduced (>50%) at 75 C. The degree of nucleotide sequence divergence indicated by these reactions suggests that these
Vibrio
species are not significantly related to
V. cholerae
or
V. parahaemolyticus
. Reassociation reactions between
V. cholerae
DNA and the DNA of
V. parahaemolyticus
indicated these species were not significantly related to each other.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
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