Author:
Ringler D H,Peter G K,Chrisp C E,Keren D F
Abstract
Antigens were extracted from a virulent isolate of Pasteurella multocida (serotype 3, 12, 15:D) with potassium thiocyanate, and a vaccine was prepared. Pasteurella-free rabbits were vaccinated intranasally and intraconjuctivally twice with a 2-week interval and challenged intranasally with the homologous P. multocida serotype 2 weeks after the second vaccination. The vaccinated rabbits produced serum immunoglobulin G and nasal mucosal immunoglobulin A against P. multocida. The vaccine protected the challenged rabbits against clinical disease and death; however, otitis media was not prevented, and microscopic inflammatory lesions were occasionally noted in the lungs and nasal turbinates. In contrast, nonvaccinated, challenged rabbits became febrile, dyspnic, depressed, and anorectic, and five of six died within 4 days of challenge with severe lesions including pneumonia, pleuritis, otitis media, and bacteremia. The vaccine prevented death and colonization of challenge organisms in the blood and lung, but did not prevent colonization of the middle ear. The vaccine alone did not cause clinical disease or gross lesions, but did produce microscopic pulmonary inflammatory lesions.
Publisher
American Society for Microbiology
Subject
Infectious Diseases,Immunology,Microbiology,Parasitology
Reference31 articles.
1. Respiratory infection in the rabbit. An enzootic caused by Pasteurella lepiseptica and attempts to control it by vaccination;Alexander M. M.;J. Infect. Dis.,1952
2. Studies on the somatic and capsular antigens of Pasteurella multocida as protection inducing factors in rabbits;Bapat J. A.;Ind. J. Pathol. Bacteriol.,1972
3. Antigenic analysis of Pasteurella multocida (serotype 1) by crossed immunoelectrophoresis: characterization of whole cell associated antigens;Bhasin J. L.;Can. J. Microbiol.,1980
4. Lysates of turkeygrown Pasteurella multocida: effects of solubilizing agents on the immunogenic properties of membrane vesicles;Brogden K. A.;Am. J. Vet. Res.,1983
5. Immune responses of rabbits, mice and sheep to polyvalent Pasteurella multocida vaccine;Cameron C. M.;Onderstepoort J. Vet. Res.,1970
Cited by
25 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献