Relationship between Pyrazinamide Resistance, Loss of Pyrazinamidase Activity, and Mutations in the pncA Locus in Multidrug-Resistant Clinical Isolates of Mycobacterium tuberculosis-Reference-Cited by-同舟云学术

Relationship between Pyrazinamide Resistance, Loss of Pyrazinamidase Activity, and Mutations in the pncA Locus in Multidrug-Resistant Clinical Isolates of Mycobacterium tuberculosis

Published:1999-09 Issue:9 Volume:43 Page:2317-2319
ISSN:0066-4804
Container-title:Antimicrobial Agents and Chemotherapy
language:en
Short-container-title:Antimicrob Agents Chemother

Author:

Mestdagh M.¹,Fonteyne P. A.²,Realini L.²,Rossau R.³,Jannes G.³,Mijs W.³,De Smet K. A. L.³,Portaels F.²,Van den Eeckhout E.¹

Affiliation:

1. Laboratory for Pharmaceutical Biotechnology, FFW, University of Ghent, 9000 Ghent,1

2. Unit of Mycobacteriology, Institute of Tropical Medicine, 2000 Antwerp,2 and

3. Innogenetics n.v., 9052 Ghent,3 Belgium

Abstract

ABSTRACT Sixty-two Mycobacterium tuberculosis isolates were tested for pyrazinamidase activity, and their pyrazinamide susceptibility was determined by the radiometric method. Sequencing of pncA genes in the 23 resistant strains revealed mutations in 16 pyrazinamidase-negative strains, 11 of which had not been previously described. Six isolates containing wild-type pncA might possess alternative resistance mechanisms.

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Pharmacology (medical),Pharmacology

Link

https://journals.asm.org/doi/pdf/10.1128/AAC.43.9.2317

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