Optimization of Culture of Leptospira from Humans with Leptospirosis

Author:

Wuthiekanun Vanaporn1,Chierakul Wirongrong12,Limmathurotsakul Direk1,Smythe Lee D.3,Symonds Meegan L.3,Dohnt Michael F.3,Slack Andrew T.3,Limpaiboon Roongreung4,Suputtamongkol Yupin5,White Nicholas J.16,Day Nicholas P. J.16,Peacock Sharon J.16

Affiliation:

1. Mahidol-Oxford Tropical Medicine Research Unit, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand

2. Department of Clinical Tropical Medicine, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand

3. WHO/FAO/OIE Collaborating Centre for Reference and Research on Leptospirosis, Centre for Public Health Sciences, Queensland Health Scientific Services, Brisbane, Australia

4. Medical Department, Udon Thani General Hospital, Udon Thani, Thailand

5. Department of Medicine, Faculty of Medicine, Siriraj Hospital, Mahidol University, Bangkok, Thailand

6. Centre for Clinical Vaccinology and Tropical Medicine, Nuffield Department of Clinical Medicine, University of Oxford, Churchill Hospital, Oxford OX3 7LJ, United Kingdom

Abstract

ABSTRACT A prospective study of 989 patients with acute febrile illness was performed in northeast Thailand to define the yield of Leptospira from four different types of blood sample. Based on a comparison of the yields from whole blood, surface plasma, deposit from spun plasma, and clotted blood samples from 80 patients with culture-proven leptospirosis, we suggest a sampling strategy in which culture is performed using whole blood and deposit from spun plasma.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Reference10 articles.

1. Doungchawee, G., D. Phulsuksombat, P. Naigowit, Y. Khoaprasert, N. Sangjun, S. Kongtim, and L. Smythe. 2005. Survey of leptospirosis of small mammals in Thailand. Southeast Asian J. Trop. Med. Public Health36:1516-1522.

2. Ellinghausen, H. C., Jr., and W. G. McCullough. 1965. Nutrition of Leptospira pomoa and growth of 13 other serotypes: fractionation of oleic albumin complex and a medium of bovine albumin and polysorbate 80. Am. J. Vet. Res.26:45-51.

3. Faine S. B. Adler C. Bolin and P. Perolat (ed.). 1999. Leptospira and leptospirosis 2nd ed. MediSci Melbourne Australia.

4. Griffith, M. E., L. L. Horvath, W. V. Mika, J. S. Hawley, J. E. Moon, D. R. Hospenthal, and C. K. Murray. 2006. Viability of Leptospira in BacT/ALERT MB media. Diagn. Microbiol. Infect. Dis.54:263-266.

5. Leptospirosis

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