Generation of False-Positive SARS-CoV-2 Antigen Results with Testing Conditions outside Manufacturer Recommendations: A Scientific Approach to Pandemic Misinformation-Reference-Cited by-同舟云学术

Generation of False-Positive SARS-CoV-2 Antigen Results with Testing Conditions outside Manufacturer Recommendations: A Scientific Approach to Pandemic Misinformation

Published:2021-10-31 Issue:2 Volume:9 Page:
ISSN:2165-0497
Container-title:Microbiology Spectrum
language:en
Short-container-title:Microbiol Spectr

Author:

Patriquin Glenn¹²,Davidson Ross J.¹²³⁴,Hatchette Todd F.¹²³⁴,Head Breanne M.⁵,Mejia Edgard⁵,Becker Michael G.⁵^ORCID,Meyers Adrienne⁵,Sandstrom Paul⁵,Hatchette Jacob⁶,Block Ava⁶,Smith Nicole⁶,Ross John⁶,LeBlanc Jason J.¹²³⁴^ORCID

Affiliation:

1. Division of Microbiology, Department of Pathology and Laboratory Medicine, Nova Scotia Health (NSH), Halifax, Nova Scotia, Canada

2. Department of Pathology, Dalhousie University, Halifax, Nova Scotia, Canada

3. Department of Medicine (Infectious Diseases), Dalhousie University, Halifax, Nova Scotia, Canada

4. Department of Microbiology and Immunology, Dalhousie University, Halifax, Nova Scotia, Canada

5. National Microbiology Laboratory (NML), Public Health Agency of Canada (PHAC), Winnipeg, Manitoba, Canada

6. Praxes Medical Group, Halifax, Nova Scotia, Canada

Abstract

With the Panbio severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) antigen test being deployed in over 120 countries worldwide, understanding conditions required for its ideal performance is critical. Recently on social media, this kit was shown to generate false positives when manufacturer recommendations were not followed.

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Cell Biology,Microbiology (medical),Genetics,General Immunology and Microbiology,Ecology,Physiology

Link

https://journals.asm.org/doi/pdf/10.1128/Spectrum.00683-21

Reference30 articles.

1. Tools and Techniques for Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2)/COVID-19 Detection

2. Public Health Agency of Canada (PHAC). 2020. Interim guidance on the use of rapid antigen detection tests for the identification of SARS-CoV-2 infection. https://www.canada.ca/en/public-health/services/diseases/2019-novel-coronavirus-infection/guidance-documents/use-rapid-antigen-detection-tests.html. Accessed 9 September 2021.

3. World Health Organization (WHO). 2020. Antigen-detection in the diagnosis of SARS-CoV-2 infection using rapid immunoassays. Interim guidance. https://www.who.int/publications/i/item/antigen-detection-in-the-diagnosis-of-sars-cov-2infection-using-rapid-immunoassays. Accessed 9 September 2021.

4. US Food and Drug Administration. 2020. In vitro diagnostics EUAs. https://www.fda.gov/medical-devices/coronavirus-disease-2019-covid-19-emergency-use-authorizations-medical-devices/vitro-diagnostics-euas#individual-molecular. Accessed 9 September 2021.

5. Health Canada. 2020. Authorized medical devices for uses related to COVID-19: list of authorized testing devices. https://www.canada.ca/en/health-canada/services/drugs-health-products/covid19-industry/medical-devices/authorized/list.html. Accessed 9 September 2021.

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