Reproducibility of the Ribosomal RNA Synthesis Ratio in Sputum and Association with Markers of Mycobacterium tuberculosis Burden

Author:

Musisi Emmanuel123,Dide-Agossou Christian4ORCID,Al Mubarak Reem56,Rossmassler Karen56,Ssesolo Abdul Wahab1,Kaswabuli Sylvia1,Byanyima Patrick1,Sanyu Ingvar1,Zawedde Josephine1,Worodria William1,Voskuil Martin I.78,Savic Rada M.891011ORCID,Nahid Payam8101112,Davis J. Lucian1314ORCID,Huang Laurence101115,Moore Camille M.16,Walter Nicholas D.568

Affiliation:

1. Infectious Disease Research Collaboration, Kampala, Uganda

2. Department of Biochemistry, Makerere University, Kampala, Uganda

3. Department of Medical and Biological Sciences, Infection and Global Health Division, University of St. Andrews, St. Andrews, United Kingdom

4. Department of Epidemiology, Colorado School of Public Health, Aurora, Colorado, USA

5. Rocky Mountain Regional VA Medical Center, Aurora, Colorado, USA

6. Division of Pulmonary Sciences and Critical Care Medicine, University of Colorado Anschutz Medical Campus, Aurora, Colorado, USA

7. Department of Immunology and Microbiology, University of Colorado Anschutz Medical Campus, Aurora, Colorado, USA

8. Consortium for Applied Microbial Metrics, Aurora, Colorado, USA

9. Department of Bioengineering and Therapeutic Sciences, University of California San Francisco, San Francisco, California, USA

10. Division of Pulmonary and Critical Care Medicine, University of California San Francisco, San Francisco, California, USA

11. Division of HIV, Infectious Diseases, and Global Medicine, University of California San Francisco, San Francisco, California, USA

12. UCSF Center for Tuberculosis, San Francisco, California, USA

13. Department of Epidemiology of Microbial Diseases, Yale School of Public Health, New Haven, Connecticut, USA

14. Pulmonary, Critical Care, and Sleep Medicine Section, Yale School of Medicine, New Haven, Connecticut, USA

15. Zuckerberg San Francisco General Hospital, San Francisco, California, USA

16. Division of Biostatistics and Bioinformatics, National Jewish Health, Denver, Colorado, USA

Abstract

This study takes a major next step toward practical application of a novel pharmacodynamic marker that we believe will have transformative implications for tuberculosis. This article follows our recent report in Nature Communications that an assay called the rRNA synthesis (RS) ratio indicates the treatment-shortening of drugs and regimens.

Funder

HHS | NIH | National Institute of Allergy and Infectious Diseases

U.S. Department of Veterans Affairs

Doris Duke Charitable Foundation

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Cell Biology,Microbiology (medical),Genetics,General Immunology and Microbiology,Ecology,Physiology

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