A Rapid Drug Resistance Genotyping Workflow for Mycobacterium tuberculosis, Using Targeted Isothermal Amplification and Nanopore Sequencing-Reference-Cited by-同舟云学术

A Rapid Drug Resistance Genotyping Workflow for Mycobacterium tuberculosis, Using Targeted Isothermal Amplification and Nanopore Sequencing

Published:2021-12-22 Issue:3 Volume:9 Page:
ISSN:2165-0497
Container-title:Microbiology Spectrum
language:en
Short-container-title:Microbiol Spectr

Author:

Gliddon Harriet D.¹²,Frampton Dan³,Munsamy Vanisha⁴,Heaney Jude⁵,Pataillot-Meakin Thomas⁵,Nastouli Eleni⁵,Pym Alexander S.⁴,Steyn Adrie J. C.⁴⁶,Pillay Deenan³⁴,McKendry Rachel A.¹⁷^ORCID

Affiliation:

1. London Centre for Nanotechnology, Faculty of Mathematics and Physical Sciences, University College London, London, United Kingdom

2. National Public Health Speciality Training Programme, South West, United Kingdom

3. Division of Infection and Immunity, University College London, London, United Kingdom

4. Africa Health Research Institute, Nelson R Mandela School of Medicine, University of KwaZulu-Natal, Durban, South Africa

5. Department of Virology, University College London Hospitals NHS Foundation Trust, London, United Kingdom

6. Department of Microbiology, University of Alabama at Birmingham, Birmingham, Alabama, USA

7. Division of Medicine, University College London, London, United Kingdom

Abstract

Current methods for diagnosing drug-resistant tuberculosis are time consuming, resulting in delays in patients receiving treatment and in transmission onwards. They also require a high level of laboratory infrastructure, which is often only available at centralized facilities, resulting in further delays to diagnosis and additional barriers to deployment in resource-limited settings.

Funder

UKRI | Engineering and Physical Sciences Research Council

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Cell Biology,Microbiology (medical),Genetics,General Immunology and Microbiology,Ecology,Physiology

Link

https://journals.asm.org/doi/pdf/10.1128/Spectrum.00610-21

Reference35 articles.

1. World Health Organization. 2020. Global Tuberculosis Report 2020. World Health Organization Geneva Switzerland.

2. The Review on Antimicrobial Resistance Jim O’Neill (chair). 2016. Tackling drug-resistant infections globally: final report and recommendations.

3. Prediction of Susceptibility to First-Line Tuberculosis Drugs by DNA Sequencing