COVID-19 Antibody Detection and Assay Performance Using Red Cell Agglutination

Author:

Srivastava Kshitij1,West Kamille A.1,De Giorgi Valeria1,Holbrook Michael R.2,Bovin Nicolai V.34,Henry Stephen M.4,Flegel Willy A.1ORCID

Affiliation:

1. Department of Transfusion Medicine, NIH Clinical Center, National Institutes of Health, Bethesda, Maryland, USA

2. National Institute of Allergy and Infectious Diseases (NIAID) Integrated Research Facility, National Institutes of Health, Frederick, Maryland, USA

3. Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Moscow, Russia

4. Centre for Kode Technology Innovation, School of Engineering, Computer and Mathematical Sciences, Faculty of Design and Creative Technologies, Auckland University of Technology, Auckland, New Zealand

Abstract

We recently developed a red cell based assay to detect SARS-CoV-2 antibodies in human plasma. In the current study, we show the hands-on application of this assay in a group of COVID-19 convalescent plasma donors and healthy individuals.

Funder

New Zealand Ministry of Business, Innovation & Employment

Division of Intramural Research, National Institute of Allergy and Infectious Diseases

HHS | NIH | NIH Clinical Center

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Cell Biology,Microbiology (medical),Genetics,General Immunology and Microbiology,Ecology,Physiology

Reference44 articles.

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3. Ullah Z Rahim Z. 2020. Putin says Russia has approved 'world first' Covid-19 vaccine. But questions over its safety remain. https://www.cnn.com/2020/08/11/europe/russia-coronavirus-vaccine-putin-intl/index.html. Accessed July 7 2021.

4. FDA. 2021. Pfizer-BioNTech COVID-19 vaccine. https://www.fda.gov/emergency-preparedness-and-response/coronavirus-disease-2019-covid-19/pfizer-biontech-covid-19-vaccine. Accessed July 7 2021.

5. Craven J. 2021. COVID-19 vaccine tracker. https://www.raps.org/news-and-articles/news-articles/2020/3/covid-19-vaccine-tracker. Accessed July 7 2021.

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