Affiliation:
1. Department of Food Science, North Carolina State University, Raleigh 27695-7624.
Abstract
To determine whether pediocin is produced and has effective antilisterial activity during food fermentation, six sausage fermentation trials were conducted with antibiotic-resistant, pediocin-producing (Bac+) Pediococcus acidilactici PAC 1.0 (Strr Rifr) and an isogenic pediocin-negative (Bac-) derivative used as a control. Meat was inoculated (ca. 10(5) CFU/g) with a composite of five Listeria monocytogenes strains, each electrotransformed with pGK12 (Cmr Emr). P. acidilactici and L. monocytogenes populations were selectively enumerated by plating on media with antibiotics. This study indicated that the dry sausage fermentation process can reduce L. monocytogenes populations. Effective inactivation of L. monocytogenes was observed when the pH at the end of the fermentation portion of the process was less than 4.9. Pediocin was responsible for part of the antilisterial activity during the fermentation in each of the six trials. Furthermore, inhibition of L. monocytogenes during drying was enhanced in the presence of pediocin in the three trials in which L. monocytogenes could be detected throughout the drying process. Thus, pediocin production contributed to an increase in safety during both the fermentation and drying portions of sausage manufacturing.
Publisher
American Society for Microbiology
Subject
Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology
Reference34 articles.
1. Simple and rapid method for isolating plasmid DNA from lactic streptococci;Anderson D. G.;Appl. Environ. Microbiol.,1983
2. Listeriosis outbreak associated with Mexican-style cheese-California;Anonymous;Morbid. Mortal. Weekly Rep.,1985
3. Listeriosis associated with consumption of turkey franks;Anonymous;Morbid. Mortal. Weekly Rep.,1989
4. Inhibition of Listeria monocytogenes by bacteriocinproducing Pediococcus during the manufacture of fermented semidry sausage;Berry E. D.;J. Food Prot.,1990
5. Purification, characterization and antimicrobial spectrum of a bacteriocin produced by Pediococcus acidilactici;Bhunia A. K.;J. Appl. Bacteriol.,1988
Cited by
185 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献