Rapid Differentiation between Members of the Anginosus Group and Streptococcus dysgalactiae subsp. equisimilis within Beta-Hemolytic Group C and G Streptococci by PCR-Reference-Cited by-同舟云学术

Rapid Differentiation between Members of the Anginosus Group and Streptococcus dysgalactiae subsp. equisimilis within Beta-Hemolytic Group C and G Streptococci by PCR

Published:2006-05 Issue:5 Volume:44 Page:1836-1838
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Liu Liang-Chun¹,Tsai Jui-Chang²³,Hsueh Po-Ren⁴,Teng Lee-Jene¹⁴

Affiliation:

1. Department of Clinical Laboratory Sciences and Medical Biotechnology

2. Center for Optoelectronic Biomedicine, National Taiwan University College of Medicine

3. Division of Neurosurgery, Department of Surgery, National Taiwan University Hospital, Taipei, Taiwan

4. Department of Laboratory Medicine

Abstract

ABSTRACT Based on a pair of primers developed initially for differentiating the anginosus group from other viridans streptococci, the PCR reported here can also differentiate between members of the anginosus group and Streptococcus dysgalactiae subsp. equisimilis among beta-hemolytic group C and G streptococci. The resulting 742-bp PCR product was specific for members of the anginosus group, although a smaller, nonspecific product (361 bp) was generated from S. dysgalactiae subsp. equisimilis . Restriction digestion of the amplicon with XbaI and BsmI further differentiated Streptococcus anginosus from Streptococcus constellatu s within the anginosus group.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.44.5.1836-1838.2006

Reference17 articles.

1. Chanter, N., N. Collin, N. Holmes, M. Binns, and J. Mumford. 1997. Characterization of the Lancefield group C streptococcus 16S-23S RNA gene intergenic spacer and its potential for identification and sub-specific typing. Epidemiol. Infect.118:125-135.

2. Identification of Clinically Relevant Viridans Group Streptococci by Sequence Analysis of the 16S-23S Ribosomal DNA Spacer Region

3. Cimolai, N., R. W. Elford, L. Bryan, C. Anand, and P. Berger. 1988. Do the beta-hemolytic non-group A streptococci cause pharyngitis? Rev. Infect. Dis.10:587-601.

4. Cimolai, N., L. MacCulloch, and S. Damm. 1990. The epidemiology of beta-haemolytic non-group A streptococci isolated from the throats of children over a one-year period. Epidemiol. Infect.104:119-126.

5. What Happened to the Streptococci: Overview of Taxonomic and Nomenclature Changes

Cited by 12 articles. 订阅此论文施引文献订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献

1. The Clinical View on Streptococcus anginosus Group – Opportunistic Pathogens Coming Out of Hiding;Frontiers in Microbiology;2022-07-08

2. Shotgun whole genome sequencing of drug-resistance Streptococcus anginosus strain 47S1 isolated from a patient with pharyngitis in Saudi Arabia;Journal of Infection and Public Health;2021-12

3. The molecular determination of emm genotypes in non-group a beta-hemolytic streptococci isolated from clinical samples;Journal of Clinical Medicine of Kazakhstan;2021-08-31

4. Molecular characterization and antibiotic resistance of group G streptococci in Israel: comparison of invasive, non-invasive and carriage isolates;European Journal of Clinical Microbiology & Infectious Diseases;2016-06-21

5. A Case of Lemierre's Syndrome Caused by Streptococcus anginosus Initially Identified as Group C Streptococcus;Clinical Microbiology Newsletter;2015-01