Effect of Multiple Freeze-Thaw Cycles on Detection of Measles, Mumps, and Rubella Virus Antibodies-Reference-Cited by-同舟云学术

Effect of Multiple Freeze-Thaw Cycles on Detection of Measles, Mumps, and Rubella Virus Antibodies

Published:2003-01 Issue:1 Volume:10 Page:19-21
ISSN:1556-6811
Container-title:Clinical and Vaccine Immunology
language:en
Short-container-title:Clin Vaccine Immunol

Author:

Pinsky Norman A.¹,Huddleston Jeanne M.²,Jacobson Robert M.¹³,Wollan Peter C.⁴,Poland Gregory A.¹²

Affiliation:

1. Mayo Vaccine Research Group

2. Department of Internal Medicine

3. Department of Pediatric and Adolescent Medicine, Mayo Clinic

4. Olmsted Medical Group, Rochester, Minnesota 55905

Abstract

ABSTRACT We investigated the effect of multiple freeze-thaw cycles on mumps, measles, and rubella virus serum antibody levels with whole-virus immunoglobulin G enzyme-linked immunoassays. Fresh serum samples from nine healthy adult volunteers were divided into six sets of five aliquots each. Samples were taken through a total of 10 freeze-thaw cycles and stored at 4°C until assayed. Each assay measurement was done in replicates of five, and the mean value was reported. After completing 10 freeze-thaw cycles, we found no clinically or statistically significant effect on measured antibody levels and found no discernible detrimental effect on the ability to measure these antibodies by enzyme-linked immunoassays.

Publisher

American Society for Microbiology

Subject

Microbiology (medical),Clinical Biochemistry,Immunology,Immunology and Allergy

Link

https://journals.asm.org/doi/pdf/10.1128/CDLI.10.1.19-21.2003

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