Modified Direct Agglutination Test for Simplified Serologic Diagnosis of Leishmaniasis-Reference-Cited by-同舟云学术

Modified Direct Agglutination Test for Simplified Serologic Diagnosis of Leishmaniasis

Published:2003-09 Issue:5 Volume:10 Page:967-968
ISSN:1556-6811
Container-title:Clinical and Vaccine Immunology
language:en
Short-container-title:Clin Vaccine Immunol

Author:

Gómez-Ochoa P.¹,Castillo J. A.¹,Lucientes J.¹,Gascón M.¹,Zarate J. J.¹,Arbea J. I.¹,Larraga V.²,Rodriguez C.¹

Affiliation:

1. Departamento de Patología Animal, Facultad de Veterinaria de Zaragoza, Zaragoza

2. Centro de Investigaciones Biológicas (CSIC), Madrid, Spain

Abstract

ABSTRACT Human leishmaniasis is a severe health problem in many countries around the world. Hence, a cheap, reliable, and accurate diagnostic test is required to fight this disease. Perhaps the direct agglutination test (DAT) meets these criteria, but antigen elaboration involves many difficulties. We have developed a new antigen elaboration method, the EasyDAT method, that avoids the problems associated with the DAT. In this study, we compared the traditional DAT antigen method with our EasyDAT antigen method by using canine sera. The sensitivities (100%) and specificities (98.7%) were the same for both methods; we therefore concluded that the EasyDAT Leishmania antigen method simplifies serologic diagnosis, making this method easier and cheaper to use.

Publisher

American Society for Microbiology

Subject

Microbiology (medical),Clinical Biochemistry,Immunology,Immunology and Allergy

Link

https://journals.asm.org/doi/pdf/10.1128/CDLI.10.5.967-968.2003

Reference9 articles.

1. Abranches, P., G. Santos Gomes, N. Rachamin, L. Campino, L. F. Schnur, and C. L. Jaffe. 1991. An experimental model for canine visceral leishmaniasis. Parasite Immunol.13:537-550.

2. Boelaert, M., S. el Safi, D. Jaquet, A. de Muynck, P. van der Stuyft, and D. Le Ray. 1999. Operational validation of the direct agglutination test for diagnosis of visceral leishmaniasis. Am. J. Trop. Med. Hyg.60:129-134.

3. Boelaert, M., S. el Safi, H. Mousa, J. Githure, P. Mbati, V. L. Gurubacharya, J. Shrestha, D. Jacquet, A. de Muynck, D. Le Ray, and P. Van der Stuyft. 1999. Multicentre evaluation of repeatability and reproducibility of the direct agglutination test for visceral leishmaniasis. Trop. Med. Int. Health4:31-37.

4. Application of a direct agglutination test for detection of specific anti-Leishmania antibodies in the canine reservoir

5. el Harith, A., A. Kolk, P. A. Kager, J. Leeuwenburg, F. J. Faber, R. Muigai, S. Kingu, and J. J. Laarman. 1987. Evaluation of a newly developed direct agglutination test (DAT) for serodiagnosis and sero-epidemiological studies of visceral leishmaniasis: comparison with IFAT and ELISA. Trans. R. Soc. Trop. Med. Hyg.81:603-606.

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