Detection of Trypanosoma cruzi and Trypanosoma rangeli Infection by Duplex PCR Assay Based on Telomeric Sequences-Reference-Cited by-同舟云学术

Detection of Trypanosoma cruzi and Trypanosoma rangeli Infection by Duplex PCR Assay Based on Telomeric Sequences

Published:2003-09 Issue:5 Volume:10 Page:775-779
ISSN:1556-6811
Container-title:Clinical and Vaccine Immunology
language:en
Short-container-title:Clin Vaccine Immunol

Author:

Chiurillo Miguel Angel¹²,Crisante Gladys³,Rojas Agustina³,Peralta Andreina⁴,Dias Manuel⁴,Guevara Palmira⁴,Añez Néstor³,Ramírez José Luis²⁴

Affiliation:

1. Decanato de Medicina, Universidad Centroccidental “Lisandro Alvarado,” Barquisimeto 3001

2. Instituto de Estudios Avanzados, Ministerio de Ciencia y Tecnología, Caracas

3. Investigaciones Parasitológicas “José Francisco Torrealba,” Universidad de Los Andes, Mérida

4. Instituto de Biología Experimental, Universidad Central de Venezuela, 1041-A Caracas, Venezuela

Abstract

ABSTRACT We used the species specificity and repetitious nature of subtelomeric kinetoplastida sequences to generate a duplex PCR assay for the simultaneous detection of Trypanosoma cruzi and Trypanosoma rangeli in experimentally and naturally infected triatomine (Reduviid) bugs and in infected human subjects. The assay was species specific and was capable of detecting 1/20th of T. cruzi and 1/4th of T. rangeli cell equivalents without complementary hybridization. In addition, the PCR-based assay was robust enough for direct application to difficult biological samples such as Reduviid feces or guts and was capable of recognizing all T. cruzi and T. rangeli strains and lineages. Because the assay primers amplify entirely different target sequences, no reaction interference was observed, facilitating future adaptation of this assay to an automated format.

Publisher

American Society for Microbiology

Subject

Microbiology (medical),Clinical Biochemistry,Immunology,Immunology and Allergy

Link

https://journals.asm.org/doi/pdf/10.1128/CDLI.10.5.775-779.2003

Reference30 articles.

1. Añez, N. 1984. Studies on Trypanosoma rangeli/Tejera, 1920. VII. Its effect on the survival of infected triatomine bugs. Mem. Inst. Oswaldo Cruz79:249-255.

2. Añez, N., E. Nieves, and D. Cazorla. 1987. Studies on Trypanosoma rangeli Tejera, 1920. IX. Course of infection in different stages of Rhodnius prolixus.Mem. Inst. Oswaldo Cruz82:1-6.

3. Añez, N., G. Crisante, A. Rojas, H. Carrasco, H. Parada, Y. Yépez, R. Borges, P. Guevara, and J. L. Ramírez. 2001. Detection and significance of inapparent infection in Chagas disease in western Venezuela. Am. J. Trop. Med. Hyg.65:227-232.

4. Añez, N., H. Carrasco, H. Parada, G. Crisante, A. Rojas, N. Gonzalez, J. L. Ramírez, P. Guevara, C. Rivero, R. Borges, and J. V. Scorza. 1999. Acute Chagas' disease in western Venezuela: a clinical, seroparasitologic, and epidemiologic study. Am. J. Trop. Med. Hyg.60:215-222.

5. Batista, J. A., S. M., Texeira, J. E. Donelson, L. V. Kirchhoff, and C. M. de Sa. 1994. Characterization of a Trypanosoma cruzi poly(A)-binding protein and its genes. Mol. Biochem. Parasitol.67:301-312.

Cited by 49 articles. 订阅此论文施引文献订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献

1. Infections and Coinfections by Trypanosomatid Parasites in a Rural Community of Venezuela;Acta Parasitologica;2022-01-11

2. Validation of a novel multiplex real-time PCR assay for Trypanosoma cruzi detection and quantification in açai pulp;2020-10-15

3. Trypanosoma rangeli28Sβ Ribosomal Gene Allows Intra and Interspecific Molecular Differentiation;Vector-Borne and Zoonotic Diseases;2020-02-01

4. Regional biogeography of microbiota composition in the Chagas disease vector Rhodnius pallescens;Parasites & Vectors;2019-10-29

5. Giardia Infection and Trypanosoma Cruzi Exposure in Dogs in the Bosawás Biosphere Reserve, Nicaragua;EcoHealth;2019-08-14