Development and Evaluation of a New Triplex Immunoassay That Detects Group A Streptococcus Antibodies for the Diagnosis of Rheumatic Fever

Author:

Whitcombe Alana L.12,Hanson-Manful Paulina12,Jack Susan3,Upton Arlo4,Carr Polly Atatoa56,Williamson Deborah A.7ORCID,Baker Michael G.28,Proft Thomas12ORCID,Moreland Nicole J.12

Affiliation:

1. School of Medical Sciences, The University of Auckland, Auckland, New Zealand

2. Maurice Wilkins Centre for Biodiscovery, The University of Auckland, Auckland, New Zealand

3. Southern District Health Board, Dunedin, New Zealand

4. Southern Community Laboratories, Dunedin, New Zealand

5. Waikato District Health Board, Hamilton, New Zealand

6. Waikato University, Hamilton, New Zealand

7. Peter Doherty Institute for Infection and Immunity, Melbourne, VIC, Australia

8. Department of Public Health, University of Otago, Wellington, New Zealand

Abstract

Streptococcal serology is a cornerstone in the diagnosis of acute rheumatic fever (ARF), a postinfectious sequela associated with group A Streptococcus infection. Current tests that measure anti-streptolysin O (ASO) and anti-DNaseB (ADB) titers require parallel processing, with their predictive value limited by the low rate of decay in antibody response. Accordingly, our objective was to develop and assess the diagnostic potential of a triplex bead-based assay, which simultaneously quantifies ASO and ADB together with titers for a third antigen, SpnA.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

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