OXA-244-Producing Escherichia coli Isolates, a Challenge for Clinical Microbiology Laboratories

Author:

Hoyos-Mallecot Yannick123,Naas Thierry1234ORCID,Bonnin Rémy A.1234,Patino Rafael4,Glaser Philippe4ORCID,Fortineau Nicolas1234,Dortet Laurent1234

Affiliation:

1. Bacteriology-Hygiene Unit, Assistance Publique-Hôpitaux de Paris, Bicêtre Hospital, Le Kremlin-Bicêtre, France

2. EA7361, Université Paris Sud, Université Paris Saclay, LabEx Lermit, Faculty of Medicine, Le Kremlin-Bicêtre, France

3. Associated French National Reference Center for Antibiotic Resistance, Le Kremlin-Bicêtre, France

4. Evolution and Ecology of Resistance to Antibiotics Unit, Institut Pasteur-Assistance Publique-Hôpitaux de Paris-Université Paris Sud, Paris, France

Abstract

ABSTRACT OXA-244 is a single-point-mutant derivative of OXA-48 displaying reduced carbapenemase activity. Here, we report the microbiological features of seven OXA-244-producing Escherichia coli isolates. Only one isolate grew on ChromID Carba Smart medium (bioMérieux), but six of the seven isolates grew on ChromID extended-spectrum-β-lactamase (ESBL) medium (bioMérieux), as they coproduced an ESBL and/or a plasmid-encoded cephalosporinase. The production of a carbapenemase was detected in 57.1%, 71.4%, 71.4%, and 100% of the E. coli isolates using the Carba NP test, the Rapidec Carba NP test (bioMérieux), a matrix-assisted laser desorption ionization–time of flight mass spectrometry (MALDI-TOF MS) hydrolysis assay (Bruker), and the OXA-48 K-SeT assay (Coris BioConcept), respectively. Our results indicate that OXA-244-producing E. coli isolates are difficult to detect, which may lead to their silent spread.

Funder

Université Paris Sud

Agence Nationale de la Recherche

Assistance Publique-Hôpitaux de Paris

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Pharmacology (medical),Pharmacology

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