Affiliation:
1. Department of Infectious Diseases, Imperial College London, London W12 0NN, United Kingdom
Abstract
ABSTRACT
While investigating the requirement for phagosomal alkalinization in the host defense against pulmonary aspergillosis, we observed high morbidity of p47
phox
−/−
mice infected with pH-insensitive
Aspergillus nidulans
mutants despite a paucity of fungal growth. Fatal infection also resulted from a normally avirulent
p
-aminobenzoate auxotroph. This demonstrates that p47
phox
−/−
murine immunity contributes significantly to
A. nidulans
lethality. These data have wider implications for microbial virulence studies with p47
phox
−/−
mice.
Publisher
American Society for Microbiology
Subject
Infectious Diseases,Immunology,Microbiology,Parasitology
Reference22 articles.
1. Arst, H. N., Jr., and M. A. Peñalva. 2003. pH regulation in Aspergillus and parallels with higher eukaryotic regulatory systems. Trends Genet.19:224-231.
2. Babior, B. M. 1999. NADPH oxidase: an update. Blood93:1464-1476.
3. Bignell, E., S. Negrete-Urtasun, A. M. Calcagno, H. N. Arst, Jr., K. Haynes, and T. Rogers. 2005. The Aspergillus pH-responsive transcription factor PacC regulates virulence. Mol. Microbiol.55:1072-1084.
4. De Groote, M. A., U. A. Ochsner, M. U. Shiloh, C. Nathan, J. M. McCord, M. C. Dinauer, S. J. Libby, A. Vazquez-Torres, Y. Xu, and F. C. Fang. 1997. Periplasmic superoxide dismutase protects Salmonella from products of phagocyte NADPH-oxidase and nitric oxide synthase. Proc. Natl. Acad. Sci. USA94:13997-14001.
5. Dri, P., G. Presani, S. Perticarari, L. Alberi, M. Prodan, and E. Decleva. 2002. Measurement of phagosomal pH of normal and CGD-like human neutrophils by dual fluorescence flow cytometry. Cytometry48:159-166.
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