Methionine overproduction by Saccharomycopsis lipolytica-Reference-Cited by-同舟云学术

Methionine overproduction by Saccharomycopsis lipolytica

Published:1976-07 Issue:1 Volume:32 Page:125-130
ISSN:0099-2240
Container-title:Applied and Environmental Microbiology
language:en
Short-container-title:Appl Environ Microbiol

Author:

Morzycka E,Sawnor-Korszyńska D,Paszewski A,Grabski J,Raczyńska-Bojanowska K

Abstract

Six ethionine-resistant (Etr) regulatory mutants of Saccharomycopsis lipolytica Sl/1 overproducing methionine have been isolated. Five of them are also resistant to seleno-methionine. The activity of homocysteine synthase (O-acetyl-L-hormoserine-acetate lyase, adding hydrogen sulfide) is derepressed in these mutants and is not susceptible to the methionine-mediated repression. The pool of free methionine in Etr mutants is enhanced 1.5 to 18 times, and incorporation of 35S into methionine is 1.5 to 50 times higher than that in the wild strain. Neither accumulation of endogenous free methionine in Etr mutants nor the uptake of exogenous methionine is accompanied by an increase in the S-adenosylmethionine pool. This implies compartmentation of methionine metabolism in S. lipolytica.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Link

https://journals.asm.org/doi/pdf/10.1128/aem.32.1.125-130.1976

Reference15 articles.

1. Genetic analysis of mating type and alkane utilization in Saccharomycopsis lipolytica;Bassel J.;J. Bacteriol.,1973

2. A spectrophotometric method for the direct determination of cysteine in the presence of naturally occurring amino acids;Gaitonde M. E.;Biochem. J.,1967

3. Variations physiologiques de la levure au cours de la croissance sur l'acide lactique comme seule source de carbone;Galzy P.;C. R. Acad. Sci.,1957

4. The enzymic synthesis of L-cysteine in Escherichia coli and Salmonella typhimurium;Kredich N. M.;J. Biol. Chem.,1966

5. Methionine biosynthesis in Saccharomyces cerevisiae: mutations at the regulatory locus ETH 2. II. Physiological and biochemical data;Masselot M.;Mol. Gen. Genet.,1974

Cited by 34 articles. 订阅此论文施引文献订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献

1. Production of 10-methyl branched fatty acids in yeast;Biotechnology for Biofuels;2021-01-07

2. White Biotechnology for Amino Acids;Industrial Biorefineries & White Biotechnology;2015

3. Characterization of isolated yeast growth response to methionine analogs;Journal of Environmental Science and Health, Part B;2013-12-02

4. Biochemical principles of the use of yeast biomass and lab starter cultures in food production;Acta Alimentaria;2009-11-01

5. Production of l-methionine by submerged fermentation: A review;Enzyme and Microbial Technology;2005-06