Author:
Apel W A,Dugan P R,Filppi J A,Rheins M S
Abstract
An indirect fluorescent antibody (FA) staining technique was developed for the rapid detection of Thiobacillus ferrooxidans. The specificity of the FA stain for T. ferrooxidans was demonstrated with both laboratory and environmental samples. Coal refuse examined by scanning electron microscopy exhibited a rough, porous surface, which was characteristically covered by water-soluble crystals. Significant numbers of T. ferrooxidans were detected in the refuse pores. A positive correlation between numbers of T. ferrooxidans and acid production in coal refuse in the laboratory was demonstrated with the FA technique.
Publisher
American Society for Microbiology
Subject
Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology
Reference28 articles.
1. A preparation method for the separation of 7s gamma globulin from human serum;Baumstark J. S.;Arch. Biochem. Biophys.,1964
2. Ecology of ironoxidizing bacteria in pyritic materials associated with coal;Belly R. T.;J. Bacteriol.,1974
3. Boccardy J. A. and W. M. Spaulding. 1968. Effects of surface mining on fish and wildlife in Appalachia. Report U.S. Department of Interior Bureau of Sports Fisheries and Wildlife Washington D.C.
4. Immunofluorescence approach to the study of the ecology of Thermoplasma acidophilum in coal refuse material;Bohlool B. B.;Appl. Microbiol.,1974
5. Nonspecific staining: its control in immunofluorescence examination soil;Bohlool B. B.;Science,1968
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