A High-Throughput Method for Screening for Genes Controlling Bacterial Conjugation of Antibiotic Resistance-Reference-Cited by-同舟云学术

A High-Throughput Method for Screening for Genes Controlling Bacterial Conjugation of Antibiotic Resistance

Published:2020-12-22 Issue:6 Volume:5 Page:
ISSN:2379-5077
Container-title:mSystems
language:en
Short-container-title:mSystems

Author:

Alalam Hanna¹²,Graf Fabrice E.¹²,Palm Martin¹²^ORCID,Abadikhah Marie¹,Zackrisson Martin¹³,Boström Jonas¹,Fransson Alfred¹²,Hadjineophytou Chris¹,Persson Linnéa¹,Stenberg Simon¹,Mattsson Matilda¹,Ghiaci Payam¹²,Sunnerhagen Per¹²^ORCID,Warringer Jonas¹²,Farewell Anne¹²^ORCID

Affiliation:

1. Department of Chemistry and Molecular Biology, University of Gothenburg, Gothenburg, Sweden

2. Centre for Antibiotic Resistance Research (CARe), University of Gothenburg, Gothenburg, Sweden

3. Möller Data Workflow Systems, Gothenburg, Sweden

Abstract

The rapid transmission of antibiotic resistance genes on conjugative plasmids between bacterial host cells is a major cause of the accelerating antibiotic resistance crisis. There are currently no experimental platforms for fast and cost-efficient screening of genetic effects on antibiotic resistance transmission by conjugation, which prevents understanding and targeting conjugation.

Funder

CARe

Vetenskapsrådet

Publisher

American Society for Microbiology

Subject

Computer Science Applications,Genetics,Molecular Biology,Modeling and Simulation,Ecology, Evolution, Behavior and Systematics,Biochemistry,Physiology,Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/mSystems.01226-20

Reference49 articles.

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2. Discovery, research, and development of new antibiotics: the WHO priority list of antibiotic-resistant bacteria and tuberculosis

3. WHO. 2017. Report on antibacterial agents in clinical development. WHO, Geneva, Switzerland.

4. The Pew Charitable Trusts. 2020. Tracking the global pipeline of antibiotics in development, April 2020. The Pew Charitable Trusts, Washington, DC.

5. Conjugative plasmids: vessels of the communal gene pool

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