Affiliation:
1. Department of Cell Biology and Biochemistry, Preclinical Research and Development, Astra Hässle AB, Mölndal, Sweden
Abstract
ABSTRACT
The gastrointestinal pathogen
Helicobacter pylori
requires supplementation with either fetal calf serum (FCS), bovine serum albumin (BSA), or (2,6-dimethyl)-β-cyclodextrin (CD) for growth in a complex or defined medium. Because the availability of medium in which all components were chemically defined would facilitate metabolic studies of
H. pylori
, growth of the type strain, ATCC 43504, was compared in a defined medium with different growth additives. The dependency of
H. pylori
growth on FCS or BSA in a defined medium could partially be replaced by dependency on CD and cholesterol when the last two components were both added to the defined medium. Growth and cell yield were not affected by the addition of glucose, but the culture viability (numbers of CFU per milliliter was extended. Because therapeutic antifoams are used to relieve gastrointestinal symptoms we studied whether the unique susceptibility of
H. pylori
to the emulsifier polyoxyethylene-20-stearylether (Brij 78) was growth dependent or medium specific. The bactericidal activity exerted in buffer at pH 5 was independent of the preculture medium, and a 5-h exposure of the bacteria to 1.28 to 2.56 μg of Brij 78 per ml reduced the numbers of viable bacteria by >5 log
10
. The MICs (0.16 to 0.32 μg/ml) were lower than the corresponding minimal bactericidal concentrations in different growth media and were affected by FCS or BSA. In conclusion, CD plus cholesterol promotes the growth of
H. pylori
in a serum-free defined medium in which glucose enhances cell viability. The antibacterial activity exerted by Brij 78 is neither growth dependent nor medium specific.
Publisher
American Society for Microbiology
Cited by
26 articles.
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