Affiliation:
1. Department of Agricultural Chemistry, Faculty of Agriculture, Tohoku University, Sendai, Japan
Abstract
Nutritional characteristics of
Selenomonas ruminantium
var.
lactilytica
isolated from a sheep rumen were studied. The organism required for growth the addition of a clarified rumen fluid to a Trypticase-yeast extract medium with either lactate or glucose as an energy source. The requirement for rumen fluid was found to be satisfied by volatile fatty acids in glucose media and by biotin in lactate media. Straight-chain saturated fatty acids with C
3
to C
10
carbon skeleton had been found to be effective. Among them,
n
-valerate was most effective at the lowest concentration. An abnormal morphology was observed with
n
-valerate-deficient glucose media.
n
-Valerate was essential in glucose media, and it was stimulatory in lactate media. Fermentation products from glucose were lactate, propionate, and acetate, and fermentation products from lactate were propionate and acetate. When cells were grown in a glucose medium containing
n
-valerate-
C
14
, the label was present in cell fractions. Almost all of the activity was found in lipid materials.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
Cited by
58 articles.
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