Usefulness of Toxoplasma gondii -Specific Recombinant Antigens in Serodiagnosis of Human Toxoplasmosis-Reference-Cited by-同舟云学术

Usefulness of Toxoplasma gondii -Specific Recombinant Antigens in Serodiagnosis of Human Toxoplasmosis

Published:2004-04 Issue:4 Volume:42 Page:1779-1781
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Pietkiewicz H.¹,Hiszczyńska-Sawicka E.²,Kur J.²,Petersen E.³⁴,Nielsen H. V.⁴,Stankiewicz M.⁵,Andrzejewska I.⁶,Myjak P.¹

Affiliation:

1. Department of Tropical Parasitology, Interfaculty Institute of Maritime and Tropical Medicine, Medical University of Gdańsk, 81-519 Gdynia

2. Department of Microbiology, Gdańsk University of Technology, 80-952 Gdańsk

3. Department of Biology and Medical Parasitology, Medical University of Poznań, 87-100 Poznań, Poland

4. Laboratory of Parasitology, Statens Seruminstitut, DK-2300 Copenhagen, Denmark

5. Animal and Food Sciences Division, Lincoln University, Canterbury, New Zealand

6. Department of Laboratory Medicine, Karolinska Institute, S14186 Stockholm, Sweden

Abstract

ABSTRACT Toxoplasma gondii SAG1, GRA1, and GRA7 recombinant antigens may be regarded as tools for the detection of T. gondii immunoglobulin G antibodies in persons with chronic and acute toxoplasmosis. GRA7 is more correlated with acute toxoplasmosis. A combination of these antigens will increase the sensitivity of enzyme-linked immunosorbent assays.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.42.4.1779-1781.2004

Reference6 articles.

1. Recombinant Antigens To Detect Toxoplasma gondii -Specific Immunoglobulin G and Immunoglobulin M in Human Sera by Enzyme Immunoassay

2. Goldman, M. 1966. Evaluation of a fluorescent antibody test for amebiasis using two widely differing amoeba strains as antigen. Am. J. Trop. Med.15:694-700.

3. Hiszczyńska-Sawicka, E., A. Brillowska-Dąbrowska, S. Dąbrowski, H. Pietkiewicz, P. Myjak, and J. Kur. 2003. High yield expression and single-step purification of Toxoplasma gondii SAG1, GRA1, and GRA7 antigens in Escherichia coli.Protein Expr. Purif.27:150-157.

4. Evaluation of Recombinant Dense Granule Antigen 7 (GRA7) of Toxoplasma gondii for Detection of Immunoglobulin G Antibodies and Analysis of a Major Antigenic Domain

5. Johnson, A. M., H. Roberts, and A. M. Tenter. 1992. Evaluation of a recombinant antigen ELISA for the diagnosis of acute toxoplasmosis and comparison with traditional ELISAs. J. Med. Microbiol.37:404-409.

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