Immunological Characterization of the Spike Protein of the Severe Acute Respiratory Syndrome Coronavirus
-
Published:2004-04
Issue:4
Volume:42
Page:1570-1576
-
ISSN:0095-1137
-
Container-title:Journal of Clinical Microbiology
-
language:en
-
Short-container-title:J Clin Microbiol
Author:
Lu Liqun1, Manopo Ivanus1, Leung Bernard P.2, Chng Hiok Hee2, Ling Ai Ee3, Chee Li Lian1, Ooi Eng Eong4, Chan Shzu-Wei1, Kwang Jimmy1
Affiliation:
1. Animal Health Biotechnology Unit, Temasek Life Science Laboratory, National University of Singapore 2. Department of Rheumatology, Allergy, and Immunology, Tan Tock Seng Hospital 3. Virology Section, Department of Pathology, Singapore General Hospital 4. Environment Health Institute, National Environment Agency, Singapore
Abstract
ABSTRACT
Severe acute respiratory syndrome (SARS) is a novel infectious disease caused by the SARS-associated coronavirus (SARS-CoV). There are four major structural proteins in the SARS-CoV, including the nucleocapsid, spike, membrane, and small envelope proteins. In this study, two sets of truncated fragments of spike protein were generated, the first were approximately 210-bp nonoverlapping fragments and the second were overlapping segments of 750 to 900 bp. From these 23 fragments, we identified a fragment of 259 amino acids (amino acids 441 to 700) that is a major immunodominant epitope. This fragment was highly expressed, and the purified fragment C could detect all 33 SARS patient serum samples tested, collected from 7 to 60 days after the onset of fever, but had no reactivity with all 66 healthy human serum samples tested. Thus, fragment C of spike protein was identified as an immunodominant antigen and could be used for serological detection of SARS-CoV infection.
Publisher
American Society for Microbiology
Subject
Microbiology (medical)
Reference28 articles.
1. Callebaut, P., L. Enjuanes, and M. Pensaert. 1996. An adenovirus recombinant expressing the spike glycoprotein of porcine respiratory coronavirus is immunogenic in swine. J. Gen. Virol.77:309-313. 2. Drosten, C., S. Gunther, W. Preiser, S. van der Werf, H. R. Brodt, S. Becker, H. Rabenau, M. Panning, L. Kolesnikova, R. A. Fouchier, A. Berger, A. M. Burguiere, J. Cinatl, M. Eickmann, N. Escriou, K. Grywna, S. Kramme, J. C. Manuguerra, S. Muller, V. Rickerts, M. Sturmer, S. Vieth, H. D. Klenk, A. D. Osterhaus, H. Schmitz, and H. W. Doerr. 2003. Identification of a novel coronavirus in patients with severe acute respiratory syndrome. N. Engl. J. Med.348:1967-1976. 3. Enjuanes, L., et al. 2000. Coronaviridae, p. 845-849. In M. H. V. van Regenmortal, C. M. Fauqet, D. H. L. Bishop, E. B. Carstens, M. K. Estes, S. M. Lemon, M. A. Mayo, D. J. McGeoch, C. R. Pringle, and R. B. Wickner (ed.), Virus taxonomy. Academic Press, New York, N.Y. 4. Fouchier, R. A., T. Kuiken, M. Schutten, G. van Amerongen, G. J. van Doornum, B. G. van den Hoogen, M. Peiris, W. Lim, K. Stohr, and A. D. Osterhaus. 2003. Aetiology: Koch's postulates fulfilled for SARS virus. Nature423:240. 5. Gallagher, T. M., and M. J. Buchmeier. 2001. Coronavirus spike proteins in viral entry and pathogenesis. Virology279:371-374.
Cited by
57 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献
|
|