Anaerobic production of alginate by Pseudomonas aeruginosa: alginate restricts diffusion of oxygen

Author:

Hassett D J1

Affiliation:

1. Department of Molecular Genetics, Biochemistry and Microbiology, University of Cincinnati College of Medicine, Ohio 45257-0524, USA. hassetdj@ucbeh.san.uc.edu

Abstract

Pseudomonas aeruginosa produced alginate and elevated algD (encoding GDPmannose 6-dehydrogenase) transcription under strict anaerobic conditions, especially when using nitrate as a terminal electron acceptor. Purified alginate added to bacterial suspensions caused a decrease in growth, suggesting that alginate contributes to oxygen limitation for the organism and likely for patients afflicted with the inherited autosomal disease cystic fibrosis.

Publisher

American Society for Microbiology

Subject

Molecular Biology,Microbiology

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5. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding;Bradford M. M.;Anal. Biochem.,1976

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