Affiliation:
1. Chemical Technology Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee 37831-6194
Abstract
Resting cells of
Micrococcus luteus
have been shown to remove strontium (Sr) from dilute aqueous solutions of SrCl
2
at pH 7. Loadings of 25 mg of Sr per g of cell dry weight were achieved by cells exposed to a solution containing 50 ppm (mg/liter) of Sr. Sr binding occurred in the absence of nutrients and did not require metabolic activity. Initial binding was quite rapid (<0.5 h), although a slow, spontaneous release of Sr was observed over time. Sr binding was inhibited in the presence of polyvalent cations but not monovalent cations. Ca and Sr were bound preferentially over all other cations tested. Sr-binding activity was localized on the cell envelope and was sensitive to various chemical and physical pretreatments. Bound Sr was displaced by divalent ions or by H
+
. Other monovalent ions were less effective. Bound Sr was also removed by various chelating agents. It was concluded that Sr binding by
M. luteus
is a reversible equilibrium process. Both ion exchange mediated by acidic cell surface components and intracellular uptake may be involved in this activity.
Publisher
American Society for Microbiology
Subject
Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology
Reference16 articles.
1. Sites of metal deposition in the cell wall of Bacillus subtilis;Beveridge T. J.;J. Bacteriol.,1980
2. Removal of thorium from simulated acid process streams by fungal biomass;Gadd G. M.;Biotechnol. Bioeng.,1989
3. Affinity of various cations for Staphylococcus aureus cell wall;Galdiero G.;Experientia,1968
4. Hunt S. 1989. Diversity of biopolymer structure and its potential for ion-binding applications p. 15-48. In H. Eccles and S. Hunt (ed.) Immobilization of ions by bio-sorption. Ellis Horwood Ltd. Chichester United Kingdom.
5. Influence of divalent cations on the growth and morphology of Bacillus stearothermophilus;Jurado A. S.;J. Gen. Microbiol.,1987
Cited by
36 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献