Affiliation:
1. School of Veterinary Medicine, Tuskegee Institute, Tuskegee, Alabama
Abstract
Jenkins
, E. M. (Tuskegee Institute, Tuskegee, Ala.), A. N.
Njoku-Obi, and E. W. Adams
. Purification of the soluble hemolysins of
Listeria monocytogenes
. J. Bacteriol.
88:
418–424. 1964.—A method is described for obtaining relatively purified hemolysin preparations from both virulent and avirulent strains of
Listeria monocytogenes
. These hemolysins are protein in nature as shown by heat lability, nondialyzable properties, precipitation with trichloroacetic acid, and electrophoretic mobility. The hemolysins are antigenic in rabbits as shown by serum neutralization tests. The potency of the purified hemolysin was markedly increased by cysteine, sodium hydrosulfite, and a number of reducing agents. Many of the actions of the purified hemolysin seemed to parallel that of streptolysin O, and certain of these activities could be explained by the “thioldisulfide hypothesis.”
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
Reference6 articles.
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