Affiliation:
1. Alimentary Pharmabiotic Centre and Department of Microbiology, University College Cork, Western Road, Cork, Ireland
Abstract
ABSTRACT
The 2.1-kb cryptic plasmid pCIBAO89 from
Bifidobacterium asteroides
harbors a 1.4-kb segment which is sufficient for its autonomous replication. The segment is divided into two parts, the presumed replication origin,
ori89
, and the
rep
gene encoding the putative 41-kDa Rep89 replication initiation protein. This minimal replication region of pCIBAO89 was functionally dissected by transcriptional analyses as well as by DNA-binding studies, and the information obtained was exploited to create a number of
Escherichia coli
-
Bifidobacterium
shuttle vectors capable of transforming various bifidobacteria with an efficiency of up to 10
6
transformants/μg DNA.
Publisher
American Society for Microbiology
Subject
Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology
Cited by
30 articles.
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