Novel Epitopic Region of Glucosyltransferase B from Streptococcus mutans

Author:

Hoshino Tomonori,Kondo Yoshio,Saito Kan,Terao Yutaka,Okahashi Nobuo,Kawabata Shigetada,Fujiwara Taku

Abstract

ABSTRACTIn the development of a component vaccine against caries, the catalytic region (CAT) and glucan-binding domain (GBD) of glucosyltransferase B (GtfB) fromStreptococcus mutanshave been employed as target antigens. These regions were adopted as primary targets because they theoretically include epitopes associated with enzyme function. However, their antigenicities have not been fully evaluated. Although there are many reports about successful vaccination using these components, the principle has not yet been put to practical use. For these reasons, we came to doubt the effectiveness of the epitopes in vaccine production and reevaluated the antigenic region of GtfB by usingin silicoanalyses combined within vitroandin vivoexperiments. The results suggested that the ca. 360-amino-acid variable region (VR) in the N terminus of GtfB is more reactive than CAT and GBD. This region isS. mutansand/or GtfB specific, nonconserved among other streptococcal Gtfs, and of unknown function. Immunization using an adenovirus vector-borne DNA vaccine confirmed that VR is an epitope that shows promise for the development of a caries vaccine.

Publisher

American Society for Microbiology

Subject

Microbiology (medical),Clinical Biochemistry,Immunology,Immunology and Allergy

Reference50 articles.

1. Peptide sequences for sucrose splitting and glucan binding within Streptococcus sobrinus glucosyltransferase (water-insoluble glucan synthetase);Abo;J. Bacteriol.,1991

2. Glucan-binding proteins of the oral streptococci;Banas;Crit. Rev. Oral Biol. Med.,2003

3. Mucosal and systemic responses to an oral liposome-Streptococcus mutans carbohydrate vaccine in humans;Childers;Reg. Immunol.,1990

4. Humans immunized with Streptococcus mutans antigens by mucosal routes;Childers;J. Dent. Res.,2002

5. Nasal immunization of humans with dehydrated liposomes containing Streptococcus mutans antigen;Childers;Oral Microbiol. Immunol.,1997

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3