Cloning and expression of the ltf gene of bacteriophage T5

Abstract

The unique 5-kilobase BamHI fragment of bacteriophage T5 was cloned into plasmid pBR322. Location of the intact ltf gene on the cloned fragment was demonstrated by complementation of the ltf mutation of phage T5hd-2, identification of a plasmid-coded polypeptide of the same molecular weight as the polypeptide forming the L-shaped tail fibers, which binds to anti-T5 antibodies; and analyses of transposon Tn1000 insertions.