Author:
Bucher D J,Kharitonenkov I G,Zakomirdin J A,Grigoriev V B,Klimenko S M,Davis J F
Abstract
M-protein from influenza virus vaccine was purified by sodium dodecyl sulfate-gel chromatography and incorporated into liposomes by solubilization with octylglucoside and subsequent dialysis. Liposomes containing M-protein formed a distinct population with a density of 1.22 g/ml on sucrose-gradient centrifugation, regardless of the net charge on the liposomes. Treatment of the liposomes by freeze-fracture followed by electron microscopic examination showed multilamellar structures in those liposomes without M-protein; liposomes containing M-protein were mulberry-like structures which appeared unilamellar. These studies show incorporation of M-protein into the lipid bilayer.
Publisher
American Society for Microbiology
Subject
Virology,Insect Science,Immunology,Microbiology
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