Affiliation:
1. CSIRO Molecular Science, Sydney Laboratory, North Ryde, New South Wales 1670,1 and
2. School of Biological Sciences, Macquarie University Sydney, New South Wales 2109,2 Australia
Abstract
ABSTRACT
The class 1 integron integrase, IntI1, recognizes two distinct types of recombination sites,
attI
sites, found in integrons, and members of the 59-be family, found in gene cassettes. The efficiencies of the integrative version of the three possible reactions, i.e., between two 59-be, between
attI1
and a 59-be, or between two
attI1
sites, were compared. Recombination events involving two
attI1
sites were significantly less efficient than the reactions in which a 59-be participated, and the
attI1
× 59-be reaction was generally preferred over the 59-be × 59-be reaction. Recombination of
attI1
with secondary sites was less efficient than the 59-be × secondary site reaction.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
Cited by
83 articles.
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