Affiliation:
1. Department of Biochemistry and Microbiology, University of Victoria, Victoria, British Columbia, Canada V8W 3P6
Abstract
ABSTRACT
The direct interaction of the
Escherichia coli
cytotoxin RelE with its specific antidote, RelB, was demonstrated in two ways: (i) copurification of the two proteins and (ii) a positive yeast two-hybrid assay involving the
relB
and
relE
genes. In addition, the purified RelE protein exhibited ribosome-binding activity in an in vitro assay, supporting previous observations suggesting that it is an inhibitor of translation.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
Reference17 articles.
1. Ausubel
F. M.
Brent
R.
Kingston
R. E.
Moore
D. D.
Seidman
J. G.
Smith
J. A.
Struhl
K.
Current protocols in molecular biology.
1994
Greene Publishing Associates & Wiley Interscience
New York N.Y
2. Sequence of the relB transcription unit from Escherichia coli and identification of the relB gene;Bech F. W.;EMBO J.,1985
3. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding
4. Cashel
M.
Gentry
D. R.
Hernandez
V. J.
Vinella
D.
The stringent response
Escherichia coli and Salmonella: cellular and molecular biology
2nd ed.
Neidhardt
F. C.
1996
1458
1496
ASM Press
Washington D.C.
5. Variations in phenotype of relB mutants of Escherichia coli and the effect of pus and sup mutations;Diderichsen B.;Mol. Gen. Genet.,1980
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