Anaerobic Growth and Denitrification among Different Serogroups of Soybean Rhizobia

Author:

van Berkum Peter1,Keyser Harold H.1

Affiliation:

1. Nitrogen Fixation and Soybean Genetics Laboratory, Agricultural Research Service, U.S. Department of Agriculture, Beltsville, Maryland 20705

Abstract

We screened soybean rhizobia originating from three germplasm collections for the ability to grow anaerobically in the presence of NO 3 and for differences in final product formation from anaerobic NO 3 metabolism. Denitrification abilities of selected strains as free-living bacteria and as bacteroids were compared. Anaerobic growth in the presence of NO 3 was observed in 270 of 321 strains of soybean rhizobia. All strains belonging to the 135 serogroup did not grow anaerobically in the presence of NO 3 . An investigation with several strains indicated that bacteria not growing anaerobically in the presence of NO 3 also did not utilize NO 3 as the sole N source aerobically. An exception was strain USDA 33, which grew on NO 3 but failed to denitrify. Dissimilation of NO 3 by the free-living cultures proceeded without the significant release of intermediate products. Nitrous oxide reductase was inhibited by C 2 H 2 , but preceding steps of denitrification were not affected. Final products of denitrification were NO 2 , N 2 O, or N 2 ; serogroups 31, 46, 76, and 94 predominantly liberated NO 2 , whereas evolution of N 2 was prevalent in serogroups 110 and 122, and all three were formed as final products by strains belonging to serogroups 6 and 123. Anaerobic metabolism of NO 3 by bacteroid preparations of Bradyrhizobium japonicum proceeded without delay and was evident by NO 2 accumulation irrespective of which final product was formed by the strain as free-living bacteria. Anaerobic C 2 H 2 reduction in the presence of NO 3 was observed in bacteroid preparations capable of NO 3 respiration but was absent in bacteria that were determined to be deficient in dissimilatory nitrate reductase.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Reference26 articles.

1. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding;Bradford M. M.;Anal. Biochem.,1976

2. Anaerobic-nitrate, symbiotic and aerobic growth of Rhizobium japonicum: effects on cytochrome P450, other haemproteins, nitrate and nitrite reductases;Daniel R. M.;Biochim. Biophys. Acta,1972

3. Anaerobic growth, nitrate reduction and denitrification in 46 Rhizobium strains;Daniel R. M.;J. Gen. Microbiol.,1982

4. Diphosphopyridine nucleotide-nitrate reductase from soybean nodules;Evans H. J.;Plant Physiol.,1954

5. Preparatjon of nitrogenase from nodules and separation into components;Evans H. J.;Methods Enzymol.,1972

Cited by 41 articles. 订阅此论文施引文献 订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3