Molecular Characterization of Cryptosporidium spp. from Children in Kolkata, India

Author:

Das Pradeep1,Roy Seuli Saha2,MitraDhar Kakali2,Dutta Phalguni2,Bhattacharya Mihir K.2,Sen Abhik2,Ganguly Sandipan2,Bhattacharya Sujit K.2,Lal Altaf A.3,Xiao Lihua3

Affiliation:

1. Rajendra Memorial Research Institute of Medical Sciences, Agam Kuan, Patna 800 007, India

2. National Institute of Cholera and Enteric Diseases, P-33, C.I.T. Road, Scheme-XM, Beliaghata, Kolkata 700 010, India

3. National Center for Infectious Diseases, Centers for Disease Control and Prevention, Building 22, Mail Stop F-12, 4770 Buford Highway, Atlanta, Georgia 30334

Abstract

ABSTRACT The intracellular parasite Cryptosporidium is responsible for severe diarrhea in immunocompromised persons in developing countries. Few studies on the characterization of the parasite in India are available. In this study, molecular characterization of the parasite from diarrheic children was carried out by PCR-restriction fragment length polymorphism analysis. At least three genotypes were identified. Out of 40 positive samples, 35 were positive for C. hominis , 4 were positive for C. parvum , and 1 was positive for C. felis . This study clearly suggests that cryptosporidiosis in this region is caused largely by anthroponotic transmission.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

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