Author:
Shields M S,Kline B C,Tam J E
Abstract
In Escherichia coli, concentrations of a mini-F plasmid with two origins of replication (oriV and oriS) were 50% lower in fast-growing cells than in slow-growing cells. By contrast, a mini-F plasmid deleted for oriV maintained a uniform concentration in both fast- and slow-growing cells, and in this behavior the plasmid mimicked the control by the host of chromosomal origin (oriC) concentration.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
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