Genome Sequence of the Polysaccharide-Degrading, Thermophilic Anaerobe Spirochaeta thermophila DSM 6192

Author:

Angelov Angel1,Liebl Susanne12,Ballschmiter Meike1,Bömeke Mechthild12,Lehmann Rüdiger2,Liesegang Heiko2,Daniel Rolf12,Liebl Wolfgang13

Affiliation:

1. Department of Genomic and Applied Microbiology

2. Göttingen Genomics Laboratory, Institute of Microbiology and Genetics, Georg-August-Universität, Grisebachstr. 8, D-37077 Goettingen, Germany

3. Department of Microbiology, Technische Universität München, Emil-Ramann-Str. 4, D-85354 Freising, Germany

Abstract

ABSTRACT Spirochaeta thermophila is a thermophilic, free-living anaerobe that is able to degrade various α- and β-linked sugar polymers, including cellulose. We report here the complete genome sequence of S. thermophila DSM 6192, which is the first genome sequence of a thermophilic, free-living member of the Spirochaetes phylum. The genome data reveal a high density of genes encoding enzymes from more than 30 glycoside hydrolase families, a noncellulosomal enzyme system for (hemi)cellulose degradation, and indicate the presence of a novel carbohydrate-binding module.

Publisher

American Society for Microbiology

Subject

Molecular Biology,Microbiology

Reference11 articles.

1. Aksenova, H., F. Rainey, P. Janssen, G. Zavarzin, and H. Morgan. 1992. Spirochaeta thermophila sp. nov., an obligately anaerobic, polysaccharolytic, extremely thermophilic bacterium. Int. J. Syst. Bacteriol. 42 : 175-177.

2. Eddy, S. R. 2008. A probabilistic model of local sequence alignment that simplifies statistical significance estimation. PLoS Comput. Biol. 4 : e1000069.

3. Ewing, B., and P. Green. 1998. Base-calling of automated sequencer traces using phred. II. Error probabilities. Genome Res. 8 : 186-194.

4. Ewing, B., L. Hillier, M. C. Wendl, and P. Green. 1998. Base-calling of automated sequencer traces using phred. I. Accuracy assessment. Genome Res. 8 : 175-185.

5. The Pfam protein families database

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