Author:
Brooks B O,Wassom D L,Cypess R H
Abstract
A sensitive and specific enzyme-linked immunosorbent assay was designed to measure the kinetics of Trypanosoma musculi-specific immunoglobulin M (IgM) and IgG responses in mice. Serum was obtained from congenitally athymic (nude) mice, their phenotypically normal, thymus-bearing littermates (NLM), and thymus cell-repaired nude mice (Nu-TC) at 6-day intervals throughout T. musculi parasitemia. NLM mice were shown to effect an antibody response to T. musculi that included an IgM to IgG shift and was correlated in time with reduction of parasite reproduction and stabilization of parasitemia. Nude mice were shown to effect a T. musculi-specific IgM response similar in onset and magnitude to that in NLM mice; this response was correlated in time with stabilization of parasitemia. Nu-TC mice were shown to effect IgM and IgG responses to T. musculi similar in time and magnitude to those in NLM mice. In marked contrast to NLM mice, Nu-TC mice did not exhibit suppression of T. musculi-specific IgM production after the IgM to IgG shift in response to this parasite.
Publisher
American Society for Microbiology
Subject
Infectious Diseases,Immunology,Microbiology,Parasitology
Reference21 articles.
1. Lack of immunoglobulin M suppression by immunoglobulin G antibody in thymectomized, irradiated, bone marrowreconstituted mice infected with Toxoplasma gondii;Aryanpour J.;Infect. Immun.,1980
2. The cytotoxic test for demonstration of mouse antibody;Boyse E. A.;Methods Med. Res.,1964
3. Thymus dependency of Trypanosoma musculi elimination from mice;Brooks B. O.;J. Reticuloendothel. Soc.,1977
4. The effect of trypan blue on the early control of Trypanosoma musculi parasitemia in mice;Brooks B. O.;J. Reticuloendothel. Soc.,1979
5. Absorption of ablastic activity from mouse serum by using a Trypanosoma musculi population rich in dividing forms;Brooks B. O.;Infect. Immun.,1980
Cited by
4 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献