Expression of Green Fluorescent Protein as a Marker for Effects of Antileishmanial Compounds In Vitro-Reference-Cited by-同舟云学术

Expression of Green Fluorescent Protein as a Marker for Effects of Antileishmanial Compounds In Vitro

Published:2001-12 Issue:12 Volume:45 Page:3654-3656
ISSN:0066-4804
Container-title:Antimicrobial Agents and Chemotherapy
language:en
Short-container-title:Antimicrob Agents Chemother

Author:

Kamau Sarah W.¹,Grimm Felix¹,Hehl Adrian B.¹

Affiliation:

1. Institute of Parasitology, University of Zürich, CH-8057 Zürich, Switzerland

Abstract

ABSTRACT Transgenic Leishmania infantum promastigotes, which constitutively express green fluorescent protein (GFP) in their cytoplasm, were used to monitor the effects of antileishmanial compounds in real time. The GFP-based assay provided a reliable measure of drug-induced inhibitory effects on protein expression, resulting in a dynamic picture of the responses of leishmanial promastigotes to the compounds tested.

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Pharmacology (medical),Pharmacology

Link

https://journals.asm.org/doi/pdf/10.1128/AAC.45.12.3654-3656.2001

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